CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02]

CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02]

• Description: Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
• Product Name Alternative: CD 14 antibody CD_antigen=CD14 antibody CD14 antibody CD14 antigen antibody CD14 molecule antibody CD14_HUMAN antibody LPS-R antibody Mo2 antibody Monocyte differentiation antigen CD14 antibody Monocyte differentiation antigen CD14 urinary form antibody Monocyte differentiation antigen CD14, membrane-bound form antibody Myeloid cell specific leucine rich glycoprotein antibody Myeloid cell-specific leucine-rich glycoprotein antibody
• Abbreviation: CD14
• CAS Number: 9000-83-3
• Swiss Prot: P08571 Human, P10810 Mouse
• Cellular Locus: Cell membrane, Secreted, Golgi apparatus, Membrane raft.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic peptide within Human CD14 aa 310-335 / 375.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, mIHC, FC
• Positive Control: THP-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysates, A549, NCCIT, NIH/3T3, LO2, human tonsil tissue, human liver tissue, human colon carcinoma tissue, human spleen tissue, human uterus tissue, human lymph nodes tissue, human cervical cancer.
• Concentration: 1 mg/mL
• Dilution: WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:200-1:800 ;mIHC: 1:800-1:1,000 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 40 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes