PRP19 Recombinant Rabbit Monoclonal Antibody [SC06-53]

• Description:

  Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.

• Product Name Alternative:

  hPso4 antibody NMP200 antibody Nuclear matrix protein 200 antibody Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody pre mRNA processing factor 19 antibody Pre-mRNA-processing factor 19 antibody PRP19 antibody PRP19/PSO4 homolog antibody PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody PRP19_HUMAN antibody PRPF19 antibody PSO4 antibody psoralen 4 antibody Senescence evasion factor antibody SNEV antibody UBOX4 antibody

• Abbreviation:

  hPso4, PRPF19

• Swiss Prot:

  Q9UMS4 Human, Q99KP6 Mouse, Q9JMJ4 Rat

• Cellular Locus:

  Nucleus, Cytoplasm, Lipid droplet.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  recombinant protein within Human PRP19 aa 380-524 / 504.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IF-Cell, IHC-P, FC

• Positive Control:

  Jurkat, MCF-7, Hela, B16-F1, mouse brain tissue, human lung cancer tissue, mouse kidney tissue, human breast carcinoma tissue, human kidney tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  55 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3