Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32]

Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32]

• Description: The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, are important signaling intermediates that mediate responses to a broad range of ligand-activated receptor tyrosine kinases. It has been established that Rsk-3 is not activated by MAP kinase in vitro, unlike Rsk-1 and Rsk-2. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. The Rsk family amino-terminal kinase domain is phosphorylated on Ser 227 by 3-phosphoinositide-dependent protein kinase-1 (PDK1), which increases the kinase activity of Rsk. In the carboxy-terminal kinase domain, Rsk-1 and Rsk-2 are autophosphorylated on Ser 380 and Ser 386, respectively, which mediates the docking of PDK1 to Rsk in order to promote phosphorylation of substrates, such as histone H3.
• Product Name Alternative: 90 kDa ribosomal protein S6 kinase 1 antibody dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody dJ590P13.1 antibody EC 2.7.11.1 antibody HU 1 antibody HU1 antibody KS6A1_HUMAN antibody MAP kinase activated protein kinase 1a antibody MAP kinase-activated protein kinase 1a antibody MAPK-activated protein kinase 1a antibody MAPKAP kinase 1a antibody MAPKAPK-1a antibody MAPKAPK1A antibody MGC79981 antibody Mitogen-activated protein kinase-activated protein kinase 1A antibody OTTHUMP00000004113 antibody p90 RSK1 antibody p90-RSK 1 antibody p90rsk antibody p90RSK1 antibody p90S6K antibody pp90RSK1 antibody Ribosomal protein S6 kinase 90kD 1 antibody Ribosomal protein S6 kinase 90kD polypeptide 1 antibody Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody Ribosomal protein S6 kinase alpha 1 antibody Ribosomal protein S6 kinase alpha-1 antibody Ribosomal protein S6 kinase polypeptide 1 antibody Ribosomal S6 kinase 1 antibody RPS6K1 alpha antibody rps6ka antibody Rps6ka1 antibody RSK 1 antibody RSK 1 p90 antibody RSK antibody RSK-1 antibody RSK1 antibody S6K alpha 1 antibody S6K-alpha-1 antibody
• Abbreviation: S6K-alpha-1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1, RPS6KA1
• CAS Number: 9000-83-3
• Swiss Prot: Q15418 Human, P18653 Mouse, Q63531 Rat
• Cellular Locus: Nucleus, Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding S380 of human RSK1.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IP
• Positive Control: A431 cell lysates, Hela, A431, 293, Hela whole cell lysate, Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate, NIH/3T3 whole cell lysate, NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 83 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes