Phospho-Smad3 (S423 + S425) Recombinant Rabbit Monoclonal Antibody [ST0493]

Phospho-Smad3 (S423 + S425) Recombinant Rabbit Monoclonal Antibody [ST0493]

• Description: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. Human Smad3 is subject to phosphorylation by TGFβ on specific amino acid residues, including Ser 208.
• Product Name Alternative: DKFZP586N0721 antibody DKFZp686J10186 antibody hMAD 3 antibody hMAD-3 antibody hSMAD3 antibody HSPC193 antibody HST17436 antibody JV15 2 antibody JV15-2 antibody JV152 antibody LDS1C antibody LDS3 antibody MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody MAD homolog 3 antibody Mad homolog JV15 2 antibody Mad protein homolog antibody MAD, mothers against decapentaplegic homolog 3 antibody Mad3 antibody MADH 3 antibody MADH3 antibody MGC60396 antibody Mothers against decapentaplegic homolog 3 antibody Mothers against DPP homolog 3 antibody SMA and MAD related protein 3 antibody SMAD 3 antibody SMAD antibody SMAD family member 3 antibody SMAD, mothers against DPP homolog 3 antibody Smad3 antibody SMAD3_HUMAN antibody
• Abbreviation: MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, SMAD 3, Smad3, hSMAD3
• CAS Number: 9000-83-3
• Swiss Prot: P84022 Human, Q8BUN5 Mouse
• Cellular Locus: Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P
• Positive Control: A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, HeLa starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, NIH/3T3 starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, A549, human large intestine tissue, mouse skin tissue, mouse kidney tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000-1:5,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:200 ;IHC-P: 1:200-1:500
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 48 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes