Cyclin B1 Recombinant Rabbit Monoclonal Antibody [SU33-03]

Cyclin B1 Recombinant Rabbit Monoclonal Antibody [SU33-03]

• Description: G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated. Cyclin B1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. Once activated, cyclin B1-Cdk1 promotes several of the events of early mitosis. The active complex phosphorylates and activates 13S condensin, which helps to condense chromosomes. Another important function of the cyclin B1-Cdk1 complex is to break down the nuclear envelope. Phosphorylation of the lamins by cyclin B1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. The destruction of the nuclear envelope is important because it allows the mitotic spindle to access the chromosomes.
• Product Name Alternative: CCNB 1 antibody CCNB antibody ccnb1 antibody CCNB1_HUMAN antibody Cyclin B1 antibody G2 mitotic specific cyclin B1 antibody G2/mitotic-specific cyclin-B1 antibody
• Abbreviation: CCNB1, CCNB
• CAS Number: 9000-83-3
• Swiss Prot: P14635 Human
• Cellular Locus: Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within Human Cyclin B1 aa 384-433 / 433.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
• Positive Control: HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, human tonsil tissue, human colon carcinoma tissue, human lymph nodes tissue, human breast carcinoma tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:400 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 48 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes