Phospho-JAK2 (Y1007 + Y1008) Recombinant Rabbit Monoclonal Antibody [SY24-03]

Phospho-JAK2 (Y1007 + Y1008) Recombinant Rabbit Monoclonal Antibody [SY24-03]

• Description: JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immuno-logical stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK-Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK-Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 are sites of trans- or autophosphorylation in vivo and in in vitro kinase reactions.
• Product Name Alternative: JAK 2 antibody JAK-2 antibody JAK2 antibody JAK2_HUMAN antibody Janus Activating Kinase 2 antibody Janus kinase 2 (a protein tyrosine kinase) antibody Janus kinase 2 antibody JTK 10 antibody JTK10 antibody kinase Jak2 antibody OTTHUMP00000043260 antibody THCYT3 antibody Tyrosine protein kinase JAK2 antibody Tyrosine-protein kinase JAK2 antibody
• Abbreviation: JAK-2, JAK2
• CAS Number: 9000-83-3
• Swiss Prot: O60674 Human, Q62120 Mouse, Q62689 Rat
• Cellular Locus: Nucleus, Endomembrane system, Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and 1008 of human JAK2.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, IP
• Positive Control: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate, human lung carcinoma tissue, human tonsil tissue, human kidney tissue, rat kidney tissue, mouse kidney tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:200-1:500 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 131 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes