Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4]

Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4]

• Description: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.
• Product Name Alternative: ERK 1 antibody ERK 2 antibody ERK-1 antibody ERK-2 antibody ERK1 antibody erk1/2 antibody ERK2 antibody ERT1 antibody ERT2 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal-regulated kinase 1 antibody Extracellular signal-regulated kinase 2 antibody HS44KDAP antibody HUMKER1A antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase 3 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody MAPK 3 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Microtubule-associated protein 2 kinase antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody Mitogen-activated protein kinase 3 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p41mapk antibody p42-MAPK antibody P42MAPK antibody p44-ERK1 antibody p44-MAPK antibody p44ERK1 antibody p44MAPK antibody PRKM 2 antibody PRKM1 antibody PRKM2 antibody PRKM3 antibody protein tyrosine kinase ERK2 antibody
• Abbreviation: MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAPK3, ERK1, PRKM3, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2
• CAS Number: 9000-83-3
• Swiss Prot: P27361 Human, P28482 Human, P63085 Mouse, Q63844 Mouse
• Cellular Locus: Cytoplasm, Nucleus, Membrane, Cell junction.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr185 of Human Erk2.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC, IF-Tissue
• Positive Control: Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, A549, NIH/3T3, MCF-7, human lung carcinoma tissue, human kidney tissue, human gallbladder tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100 ;IF-Tissue:1:50-1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 43/41 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes