CD68 Mouse Monoclonal Antibody [A3C4]

CD68 Mouse Monoclonal Antibody [A3C4]

• Description: CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Human CD68 is a transmembrane glycoprotein, heavily glycosylated in its extracellular domain, with a molecular weight of 110 kD. Its primary sequence consists of 354 amino acids with predicted molecular weight of 37.4 kD if it were not glycosylated. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells and myocytes. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease. Anti-CD68 monoclonal antibodies that react with tissues of rodent and other species include ED1, FA-11, KP1 (a.k.a. C68/684), 6A326, 6F3, 12E2, 10B1909, and SPM130. Monoclonals that react with humans include, Ki-M7, PG-M1, 514H12, ABM53F5, 3F7C6, 3F7D3, Y1/82A, EPR20545, CDLA68-1, LAMP4-824.
• Product Name Alternative: CD 68 antibody CD68 antibody CD68 antigen antibody CD68 molecule antibody CD68_HUMAN antibody DKFZp686M18236 antibody gp11 antibody Gp110 antibody LAMP4 antibody Macrophage antigen CD68 (microsialin) antibody MACROPHAGE ANTIGEN CD68 antibody Macrosialin antibody SCARD1 antibody Scavenger receptor class D member 1 antibody
• Abbreviation: CD68
• CAS Number: 9007-83-4
• Swiss Prot: P34810 Human
• Cellular Locus: Cell membrane. Endosome membrane, lysosome membrane.
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Synthetic peptide within human CD68 aa 320-354.
• Isotype: IgG1
• Conjugation: Non-conjugated
• Type: Mouse monoclonal Antibody
• Applications: WB, IHC-P, FC, mIHC
• Positive Control: A431 cell lysate, THP-1 cell lysate, U-937 cell lysate, human tonsil tissue, human lung tissue, THP-1, human pancreatic carcinoma.
• Concentration: 2 mg/mL
• Dilution: WB:1:1,000-1:2,000 ;IHC-P:1:200-1:1,000 ;FC:1:1,000 ;mIHC: 1:3,000
• Purity: Protein G affinity purified.
• Form: Liquid
• Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 37 kDa.
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No