BRCA2 antibody

• Background:

  Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA) . Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SHFM1/DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180) .

• Synonyms:

  Breast cancer type 2 susceptibility protein|Fanconi anemia group D1 protein|BRCA2|FACD|FANCD1

• Gene ID:

  675

• UniProt:

  P51587

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Breast cancer 2, early onset

• Target:

  BRCA2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB

• Field of Research:

  Epigenetics, Cancer, Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:200-1:1000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  384 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB

• Immunogen Sequence:

  1-160aa