ELISA Buffer Set-96T
Washing buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for washing the coated plate.
Blocking buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for blocking the coated plate. The samples and detection antibodies can be prepared by 1x blocking buffer which should be 541 times dilution.
Substrate solution: Add 100μL/well to plate.
Stop solution: Add 50μL/well to plate.
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