Biotinylated Mouse PD-L1 / B7-H1 Protein, His, Avitag™ (MALS verified)

• Background:

  Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1, which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

• Description:

  Biotinylated Mouse PD-L1 Protein, His, Avitag (PDL-M82E7) is expressed from human 293 cells (HEK293) . It contains AA Phe 19 - Thr 238 (Accession # Q9EP73) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 28.3 kDa. The protein migrates as 39-54 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q9EP73

• Expression Region:

  Phe 19 - Thr 238

• Host:

  HEK293

• Target:

  PD-L1

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Mouse

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Anti-PD-L1 Neutralizing Antibody at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-L1 Protein, His,Avitag (Cat. No. PDL-M82E7) with a linear range of 0.1-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  28.3 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Mouse