Biotinylated Cynomolgus Siglec-10 Protein, His, Avitag™ (MALS verified)

• Background:

  The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

• Description:

  Biotinylated Cynomolgus Siglec-10 Protein, His, Avitag (SI0-C82E5) is expressed from human 293 cells (HEK293) . It contains AA Thr 17 - Ala 549 (Accession # XP_005590164.1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 62.0 kDa. The protein migrates as 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  XP_005590164.1

• Expression Region:

  Thr 17 - Ala 549

• Host:

  HEK293

• Target:

  Siglec-10

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Cynomolgus

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Cynomolgus Siglec-10 Protein, His,Avitag (Cat. No. SI0-C82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Siglec-10 Antibody, Human IgG1 with a linear range of 0.02-0.625 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM MES, 150 mM NaCl, 0.5 M Arginine, pH5.0

• Additives:

  Trehalose

• Molecular Weight:

  62.0 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Cynomolgus