Cynomolgus MICA Protein, His Tag (HPLC verified)

• Background:

  MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.

• Description:

  Cynomolgus MICA Protein, His Tag (MIA-C52H5) is expressed from human 293 cells (HEK293) . It contains AA Leu 25 - Trp 304 (Accession # A0A7N9D4J5) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.9 kDa. The protein migrates as 48-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  A0A7N9D4J5

• Expression Region:

  Leu 25 - Trp 304

• Host:

  HEK293

• Target:

  MICA

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Cynomolgus

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) at 2 μg/mL (100 μL/well) can bind Cynomolgus MICA Protein, His Tag (Cat. No. MIA-C52H5) with a linear range of 0.02-0.313 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  33.9 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  500ug*2

• Species:

  Cynomolgus