Biotinylated Human HLA-A*03:01&B2M Monomer Protein (Peptide free, MALS verified)

• Background:

  Major histocompatibility complex (MHC), group of genes that code for proteins found on the surfaces of cells that help the immune system recognize foreign substances. MHC proteins are found in all higher vertebrates. In human beings the complex is also called the human leukocyte antigen (HLA) system.

• Description:

  Biotinylated Human HLA-A*03:01&B2M Monomer Protein (HLM-H82Ey) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) ) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.1 kDa and 11.7 kDa. The protein migrates as 40-45 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P04439 (HLA-A*03:01) & P61769 (B2M)

• Expression Region:

  Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M)

• Host:

  HEK293

• Target:

  HLA-A*0301 & B2M

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Human HLA-A*03:01&B2M Monomer Protein (Cat. No. HLM-H82Ey) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.06-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  36.1 kDa and 11.7 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human