Biotinylated Mouse IL-12 R beta 1 / CD212 Protein, His,Avitag™

Biotinylated Mouse IL-12 R beta 1 / CD212 Protein, His,Avitag™

• Background: The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
• Description: Biotinylated Mouse IL-12 R beta 1, His,Avitag (ILB-M52E1) is expressed from human 293 cells (HEK293). It contains AA Gln 20 - Ser 565 (Accession # Q60837-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 64.7 kDa. The protein migrates as 43-55 kDa and 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 20 - Ser 565
• Host: HEK 293
• Target: IL-12 R beta 1
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Biotinylated Mouse IL-12 R beta 1, His,Avitag (Cat. No. ILB-M52E1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-12B, Fc Tag (Cat. No. NK2-H5258) with a linear range of 0.2-8 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 64.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C