Rhesus macaque HGF R / c-MET Protein, Fc Tag

Rhesus macaque HGF R / c-MET Protein, Fc Tag

• Background: Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.
• Description: Rhesus macaque HGF R,Fc Tag (MET-R5256) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Asn 930 (Accession # G7MM61-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 127.8 kDa (α chain 32.5 kDa and β chain 95.3 kDa). The protein migrates as ＞116 kDa (alpha & beta chain), 45 kDa (α chain) and 113-116 (β chain) kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Glu 25 - Asn 930
• Host: HEK 293
• Target: HGF R
• Conjugation: Unconjugated
• Tag: C-Fc
• Source: Rhesus macaque
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Human HGF, His Tag (Cat. No. HGF-H52H3) at 2 μg/mL (100 μL/well) can bind Rhesus macaque HGF R,Fc Tag (Cat. No. MET-R5256) with a linear range of 0.1-16 ng/mL (QC tested).
• Format: Powder
• Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 32.5 kDa (α chain) & 95.3 kDa (β chain)
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C