SARS-CoV-2 Nucleocapsid protein, His Tag (B.1.1.529/Omicron)

SARS-CoV-2 Nucleocapsid protein, His Tag (B.1.1.529/Omicron)

• Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
• Description: SARS-CoV-2 Nucleocapsid protein, His Tag (B.1.1.529/Omicron) (NUN-C52Ht) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P13L, ERS31-33del, R203K, G204R)). The mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: B.1.1.529; GISAID clade: GR/484A; Nextstrain clade: 21K).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Met 1 - Ala 419
• Host: HEK 293
• Target: Nucleocapsid protein
• Conjugation: Unconjugated
• Tag: C-6×His
• Source: SARS-CoV-2
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (B.1.1.529/Omicron) (Cat. No. NUN-C52Ht) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (Cat. No. NUN-S41) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, 0.2 M Arginine, pH7.4
• Additives: Trehalose
• Molecular Weight: 47.3 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C