SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag (active enzyme)

SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag (active enzyme)

• Background: New coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Similar to the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), its genome encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus is the same as 3CLpro. This enzyme operates on no less than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential. Therefore, press this The activity of this enzyme will prevent virus imitation.
• Description: SARS-CoV-2 Papain-like Protease, His Tag (PAE-C5148) is expressed from E. coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1).
• Specifications: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 37.6 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE).
• Expression Region: Glu 746 - Lys 1060
• Host: E. coli
• Target: Papain-like Protease
• Conjugation: Unconjugated
• Tag: N-10×His
• Source: SARS-CoV-2
• Stability: The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Format: Liquid
• Buffer: 50 mM HEPES, 300 mM NaCl, pH7.5, 11% Trehalose
• Additives: Glycerol
• Molecular Weight: 37.6 kDa
• Shipping Conditions: Dry ice
• Storage Conditions: -70°C