Biotinylated Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Biotinylated Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

• Background: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
• Description: MABSol® Biotinylated Mouse CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (CDD-M82W5) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)). It is the biotinylated form of Mouse CD3 epsilon & CD3 delta Protein.
• Specifications: Biotinylated Mouse CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 17.3 kDa (CD3E) and 13.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag and followed by an Avi tag (Avitag™) and subunit CD3D contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 20-22 kDa due to glycosylation.
• Expression Region: Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D)
• Host: HEK 293
• Target: CD3E & CD3D
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi | Native
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized CD3e Monoclonal Antibody (145-2C11) at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-M82W5) with a linear range of 5-156 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 17.3 kDa (CD3E) & 13.3 kDa (CD3D)
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C