Human CD27 Ligand / CD70 Protein, Llama IgG2b Fc Tag, low endotoxin

Human CD27 Ligand / CD70 Protein, Llama IgG2b Fc Tag, low endotoxin

• Background: Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
• Description: Human CD27 Ligand, Llama IgG2b Fc Tag (CDL-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # NP_001243.1).
• Specifications: This protein carries a llama IgG2b Fc tag at the N-terminus. The protein has a calculated MW of 75.5 kDa. The protein migrates as 140-160 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ser 52 - Pro 193
• Host: HEK 293
• Target: CD27 Ligand
• Conjugation: Unconjugated
• Tag: N-LlamaFc(LlamaIgG2b)
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 0.01 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human CD27 Ligand, Llama IgG2b Fc Tag (Cat. No. CDL-H5255) at 2 μg/mL (100 μL/well) can bind Human CD27, His Tag (Cat. No. CD7-H522b) with a linear range of 4-63 ng/mL (QC tested).
• Format: Powder
• Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 75.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C