Mouse IgG3 Fc Protein, Tag Free (MALS verified)

Mouse IgG3 Fc Protein, Tag Free (MALS verified)

• Background: Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
• Description: Mouse IgG3 Fc Protein, Tag Free (IG3-M5214) is expressed from human 293 cells (HEK293). It contains AA Pro 98 - Pro 327 (Accession # P03987-2).
• Specifications: This protein carries no "tag". The protein has a calculated MW of 26.1 kDa. The protein migrates as 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Pro 98 - Pro 327
• Host: HEK 293
• Target: IgG3 Fc
• Conjugation: Unconjugated
• Tag: Native
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.6-10 ng/mL (QC tested).
• Format: Powder
• Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 26.1 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C