Rat Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free

• Background:

  Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit. It is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A and Human echoviruses 1 and 8. DGEA inhibited rotavirus binding to alpha2beta1 and infectivity. In a novel process, integrin-using viruses bind the alpha2 I domain of alpha2beta1 via DGE in VP4 and interact with alphaXbeta2 (via GPR) and alphaVbeta3 by using VP7 to facilitate cell entry and infection.

• Description:

  Rat ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-R52W9) is expressed from human 293 cells (HEK293) . It contains AA Tyr 27 - Gly 1130 (ITGA2) & Gln 21 - Asp 729 (ITGB1) (Accession # A0A0G2K470-1 (ITGA2) & P49134-1 (ITGB1) ) .

• Specifications:

  Rat ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.7 kDa (ITGB1) . Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 140-160 kDa (ITGA2) and 100-120 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  A0A0G2K470-1 (ITGA2) & P49134-1 (ITGB1)

• Expression Region:

  Tyr 27 - Gly 1130 (ITGA2) & Gln 21 - Asp 729 (ITGB1)

• Host:

  HEK293

• Target:

  Integrin alpha 2 beta 1

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis | Native

• Source:

  Rat

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Native Human Collagen IV Protein at 2 μg/mL (100 μL/well) can bind Rat ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-R52W9) with a linear range of 0.039-0.625 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH8.0

• Additives:

  Trehalose

• Molecular Weight:

  127.6 kDa (ITGA2) & 83.7 kDa (ITGB1)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Rat