Biotinylated Cynomolgus IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc, Avitag™&Fc, Avitag™ (MALS verified)

• Background:

  Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit) . The high and intermediate affinity forms also associate with a gamma subunit.

• Description:

  Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc, Avitag&Fc, Avitag (ILG-C82F6) is expressed from human 293 cells (HEK293) . It contains AA Ala 27 - Thr 240 (IL-2RB) & Glu 22 - Glu 238 (IL-2RA) & Leu 23 - Pro 255 (IL-2RG) (Accession # Q38J85-1 (IL-2RB) & A0A2K5UYC9-1 (IL-2RA) & Q38JL2-1 (IL-2RG) ) .

• Specifications:

  Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc, Avitag&Fc, Avitag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 80.0 kDa (IL-2RA&IL-2RG) . Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) . The reducing (R) protein migrates as 65-70 kDa and 115-130 kDa respectively due to glycosylation.

• Accession Number:

  Q38J85-1 (IL-2RB) & A0A2K5UYC9-1 (IL-2RA) & Q38JL2-1 (IL-2RG)

• Expression Region:

  Ala 27 - Thr 240 (IL-2RB) & Glu 22 - Glu 238 (IL-2RA) & Leu 23 - Pro 255 (IL-2RG)

• Host:

  HEK293

• Target:

  IL-2 R beta & IL-2 R alpha & IL-2 R gamma

• Conjugation:

  Biotin-labeled

• Tag:

  C-Fc & C-Avi | C-Fc & C-Avi

• Source:

  Cynomolgus

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human IL-2, His Tag (Cat. No. IL2-H52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F6) with a linear range of 8-125 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  53.0 kDa & 80.0 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Cynomolgus