Human Siglec-6 / CD327 Protein, Fc Tag

• Background:

  Sialic acid-binding Ig-like lectin 6 (SIGLEC6) is also known as CD antigen CD327, CD33 antigen-like 1 (CD33L or CD33L1), Obesity-binding protein 1 (OB-BP1) . SIGLEC6 belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family, which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIGLEC6 mediates sialic-acid dependent binding to cells. SIGLEC6 binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

• Description:

  Human Siglec-6, Fc Tag (SI6-H5256) is expressed from human 293 cells (HEK293) . It contains AA Gln 16 - Val 320 (Accession # AAH35359) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.3 kDa. The protein migrates as 80-100 kDa under reducing (R) condition, and 130-150 kDa and 250-300 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  AAH35359

• Expression Region:

  Gln 16 - Val 320

• Host:

  HEK293

• Target:

  Siglec-6

• Conjugation:

  Unconjugated

• Tag:

  C-Fc

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human Siglec-6, Fc Tag (Cat. No. SI6-H5256) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Siglec-6 Antibody, Rabbit IgG with a linear range of 0.2-16 ng/mL (Routinely tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  60.3 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  250ug*4

• Species:

  Human