Human Siglec-7 / CD328 Protein, His Tag (MALS verified)

• Background:

  Siglec-7 is a member of the human CD33‐related Siglec receptor. The extracellular region of Siglec‐7 is characterized by an N‐terminal V‐set Ig domain that can bind sialic acid and two C2‐set Ig domains. The cytoplasmic tail of Siglec‐7 has one immune‐receptor tyrosine‐based inhibitory motif (ITIM) and one ITIM‐like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.

• Description:

  Human Sigelc-7, His Tag (SG7-H5225) is expressed from human 293 cells (HEK293) . It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.8 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q9Y286-1

• Expression Region:

  Gln 19 - Leu 353

• Host:

  HEK293

• Target:

  Siglec-7

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human Sigelc-7, His Tag (Cat. No. SG7-H5225) at 5 μg/mL (100 μL/well) can bind Neu5Aca2-6GalNAca-PAA-biotin with a linear range of 6-100 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  25 mM MES, 150 mM NaCl, pH5.5

• Additives:

  Trehalose

• Molecular Weight:

  38.8 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Human