Human CD44 Protein, Fc Tag (HPLC-verified)

Human CD44 Protein, Fc Tag (HPLC-verified)

• Background: CD44 antigen is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion and migration. CD44 is expressed in a large number of mammalian cell types. CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). CD44 function is controlled by its posttranslational modifications. One critical modification involves discrete sialofucosylations rendering the selectin-binding glycoform of CD44 called HCELL (for Hematopoietic Cell E-selectin/L-selectin Ligand). CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms.
• Description: Human CD44 Protein, Fc Tag (PG1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Pro 220 (Accession # AAH04372).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 48.7 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 21 - Pro 220
• Host: HEK 293
• Target: CD44
• Conjugation: Unconjugated
• Tag: C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind Human CD44 Protein, Fc Tag (Cat. No. PG1-H5255) with a linear range of 0.156-2.5 μg/mL (Routinely tested).
• Format: Powder
• Buffer: Tris with Glycine, Arginine and NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 48.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C