Human KRAS Protein, His Tag

Human KRAS Protein, His Tag

• Background: KRAS (Kirsten rat sarcoma 2 viral oncogene homolog) gene is a proto-oncogene that encodes a small GTPase transductor protein called KRAS. KRAS is also known as Ki-Ras, c-K-ras and c-Ki-ras. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation, promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. RAS is one of the most frequently mutated oncogenes in human cancer but the frequency and distribution of RAS gene mutations are not uniform. In details, mutation of glycine 12 (G12) causes RAS activation by interfering with GAP binding and GAP-stimulated GTP hydrolysis. The reference shows the pathway may as a potential therapy targets.
• Description: Human KRAS, His Tag (KRS-H5547) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Lys 169 (Accession # P01116-2).
• Specifications: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.3 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Met 1 - Lys 169
• Host: Insect Cells
• Target: KRAS
• Conjugation: Unconjugated
• Tag: N-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: 50 mM Tris, 0.5 M NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 21.3 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C