Human G-CSF R / CD114 Protein, His Tag

• Background:

  Granulocyte Colony Stimulating Factor Receptor (G-CSFR) is also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I membrane protein has a composite structure consisting of an immunoglobulin (Ig) -like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.

• Description:

  Granulocyte Colony Stimulating Factor Receptor (G-CSFR) is also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I membrane protein has a composite structure consisting of an immunoglobulin (Ig) -like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 67.4 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  NP_000751.1

• Expression Region:

  Glu 25 - Pro 621

• Host:

  HEK293

• Target:

  G-CSF R

• Conjugation:

  Unconjugated

• Tag:

  C-6xHis

• Source:

  Human

• Stability:

  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized ActiveMax® Human G-CSF, Tag Free (Cat. No. GCF-H5214) at 2 μg/mL (100 μL/well) can bind Human G-CSF R, His Tag (Cat. No. GCR-H5223) with a linear range of 0.01-0.313 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  67.4 kDa

• Additionnal Information:

  Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  500ug*2

• Host or Source:

  HEK293

• Species:

  Human

• Protein ID:

  NP_000751.1

• Preservative:

  Trehalose