PE-Labeled Human PD-1 / PDCD1 Protein, Fc, His Tag (recommended for neutralizing assay) (Site-specific conjugation)

• Background:

  Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

• Description:

  PE-Labeled Human PD-1, Fc, His Tag (Cat. No. PD1-HP2F2) is produced via site-specific conjugation of PE to Human PD-1, Fc, His Tag under optimal conditions with a proprietary technology. Human PD-1, Fc, His Tag is expressed from human 293 cells (HEK293) . It contains AA Leu 25 - Gln 167 (Accession # Q15116-1) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 44.7 kDa.

• Accession Number:

  Q15116-1

• Expression Region:

  Leu 25 - Gln 167

• Host:

  HEK293

• Target:

  PD-1

• Conjugation:

  PE Labeled

• Tag:

  C-Fc & C-6xHis

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Format:

  Powder

• Buffer:

  PBS, 0.5% BSA, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  44.7 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C, aviod light

• Package Size:

  50tests*1

• Species:

  Human