Human CCL2 / MCP-1 Protein, Fc Tag (MALS verified)

Human CCL2 / MCP-1 Protein, Fc Tag (MALS verified)

• Background: Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. CCL2, a member of the CC subfamily, is characterized by two adjacent cysteine residues. CCL2 displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
• Description: Human CCL2, Fc Tag (CC2-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Thr 99 (Accession # P13500-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 35.1 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 24 - Thr 99
• Host: HEK 293
• Target: CCL2
• Conjugation: Unconjugated
• Tag: C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 35.1 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C