FITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, Fc Tag

FITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, Fc Tag

• Background: GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
• Description: FITC-Labeled Human GUCY2C, Fc Tag (GUC-HF255) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 72.5 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ser 24 - Gln 430
• Host: HEK 293
• Target: GUCY2C
• Conjugation: FITC Labeled
• Tag: C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human GUCY2C, Fc Tag (Cat. No. GUC-HF255) with a linear range of 0.01-0.313 μg/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 72.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light