Human SIRP alpha / CD172a Protein, Mouse IgG1 Fc Tag (MALS verified)

Human SIRP alpha / CD172a Protein, Mouse IgG1 Fc Tag (MALS verified)

• Background: Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
• Description: Human SIRP alpha, Mouse IgG1 Fc Tag (SIA-H52A8) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).
• Specifications: This protein carries a mouse IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 63.0 kDa. The protein migrates as 70-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Glu 31 - Arg 370
• Host: HEK 293
• Target: SIRP alpha
• Conjugation: Unconjugated
• Tag: C-mFc(mIgG1)
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) with a linear range of 4-31 ng/mL (QC tested).
• Format: Powder
• Buffer: 50 mM Tris, 100 mM Glycine, pH7.5
• Additives: Trehalose
• Molecular Weight: 63.0 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C