Mouse GUCY2C / Guanylyl cyclase C Protein, His Tag (MALS verified)

Mouse GUCY2C / Guanylyl cyclase C Protein, His Tag (MALS verified)

• Background: GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
• Description: Mouse GUCY2C, His Tag (GUC-M52H3) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Met 433 (Accession # Q3UWA6-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 49.0 kDa. The protein migrates as 65-95 kD when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Val 20 - Met 433
• Host: HEK 293
• Target: GUCY2C
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 49.0 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C