Biotinylated Human GUCY2C / Guanylyl cyclase C Protein, Fc,Avitag™
CAT:
716-GUC-H82F8-25ug
Size:
25 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Biotinylated Human GUCY2C / Guanylyl cyclase C Protein, Fc,Avitag™
- Background: GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
- Description: Biotinylated Human GUCY2C, Fc,Avitag (GUC-H82F8) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).
- Specifications: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 74.1 kDa. The protein migrates as 90-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Expression Region: Ser 24 - Gln 430
- Host: HEK 293
- Target: GUCY2C
- Conjugation: Biotin-labeled
- Tag: C-Fc & C-Avi
- Source: Human
- Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Endotoxin: 0.1 EU per μg
- Purity: 90%
- Bioactivity: Immobilized Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human GUCY2C, Fc,Avitag (Cat. No. GUC-H82F8) with a linear range of 0.8-25 ng/mL (QC tested).
- Format: Powder
- Buffer: PBS, pH7.4
- Additives: Trehalose
- Molecular Weight: 74.1 kDa
- Shipping Conditions: Room Temperature
- Storage Conditions: -20°C