Human Niemann-Pick Type C2 / NPC2 Protein, His Tag

Human Niemann-Pick Type C2 / NPC2 Protein, His Tag

• Background: Niemann-Pick disease type C2 protein (NPC2) is also known as epididymal secretory protein E1 and human epididymis-specific protein 1 (HE1). NPC2 is a intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. Also,NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Furthermore,the secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.
• Description: Human NPC2, His Tag (NP2-H52H1) is expressed from human 293 cells (HEK293). It contains AA Glu 20 - Leu 151 (Accession # P61916-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.5 kDa. The protein migrates as 21 kDa and 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Glu 20 - Leu 151
• Host: HEK 293
• Target: Niemann-Pick Type C2
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 16.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C