Cynomolgus / Rhesus macaque CD47 Protein, Fc Tag

• Background:

  Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

• Description:

  Cynomolgus / Rhesus macaque CD47, Fc Tag (CD7-C5252) is expressed from human 293 cells (HEK293) . It contains AA Gln 19 - Glu 141 (Accession # F7A802-1) . In the region Gln 19 - Glu 141, the AA sequence of Cynomolgus and Rhesus macaque CD47 are homologus.

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition, and 100-120 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  F7A802-1

• Expression Region:

  Gln 19 - Glu 141

• Host:

  HEK293

• Target:

  CD47

• Conjugation:

  Unconjugated

• Tag:

  C-Fc

• Source:

  Cynomolgus / Rhesus macaque

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) at 10 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, Fc Tag (Cat. No. CD7-C5252) with a linear range of 0.039-0.625 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  40.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  250ug*4

• Species:

  Cynomolgus / Rhesus macaque