HCoV-HKU1 (isolate N5) Spike Trimer Protein, His Tag (MALS verified)

• Background:

  It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Description:

  HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (SPN-H52H5) is the ectodomain of HCoV-HKU1 (isolate N5) spike protein which contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. The S1/S2 furin-recognition site 752-RRKRR-756 is mutated to GGSGS to generate the uncleaved protein. Proline substitutions (N1067P, L1068P) are introduced to stabilize the trimeric prefusion state of the spike protein.

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 145.5 kDa. The protein migrates as 180-210 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q0ZME7-1

• Expression Region:

  Ala 13 - Asn 1276

• Host:

  HEK293

• Target:

  Spike protein

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  HCoV-HKU1

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Format:

  Powder

• Buffer:

  0.1 M Sodium citrate, pH5.5

• Additives:

  Trehalose

• Molecular Weight:

  145.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  50ug*1

• Species:

  HCoV-HKU1