Hendra virus Glycoprotein, His Tag (MALS verified)

Hendra virus Glycoprotein, His Tag (MALS verified)

• Background: Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses discovered in the mid-to late 1990s that possess a broad host tropism and are known to cause severe and often fatal disease in both humans and animals. HeV and NiV infect host cells through the coordinated efforts of two envelope glycoproteins. The G glycoprotein attaches to cell receptors, triggering the fusion (F) glycoprotein to execute membrane fusion. G is a type II homotetrameric transmembrane protein responsible for binding to ephrinB2 or ephrinB3 (ephrinB2/B3) receptors. F is a homotrimeric type I transmembrane protein that is synthesized as a premature F0 precursor and cleaved by cathepsin L during endocytic recycling to yield the mature, disulfide-linked, F1 and F2 subunits. Upon binding to ephrinB2/B3, NiV G undergoes conformational changes leading to F triggering and insertion of the F hydrophobic fusion peptide into the target membrane. Subsequent refolding into the more stable post-fusion F conformation drives merger of the viral and host membranes to form a pore for genome delivery to the cell cytoplasm.
• Description: Hendra virus Glycoprotein, His Tag (GLN-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 71 - Ser 604 (Accession # O89343-1).
• Specifications: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 61.5 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 71 - Ser 604
• Host: HEK 293
• Target: Glycoprotein G (HeV)
• Conjugation: Unconjugated
• Tag: N-10×His
• Source: Hendra virus
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Hendra virus Glycoprotein, His Tag (Cat. No. GLN-H52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-B2 Protein, Fc tag (Cat. No. EPN-H5259) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, 0.3M Arginine, pH7.3
• Additives: Trehalose
• Molecular Weight: 61.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C