Human Vitronectin / VTN Protein, Tag Free, low endotoxin

• Background:

  Vitronectin is also known as S-protein, VN, VTN, V75. Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes. Blocking of Hic (a member of the pneumococcal surface protein C (PspC) family) by specific antiserum or genetic deletion significantly reduced pneumococcal binding to soluble and immobilised vitronectin and to Factor H, respectively. In addition, Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

• Description:

  Human Vitronectin Protein, Tag Free (VIN-H5214) is expressed from human 293 cells (HEK293) . It contains AA Asp 20 - Leu 478 (Accession # P04004) .

• Specifications:

  This protein carries no "tag". The protein has a calculated MW of 52.3 kDa. The protein migrates as 67 kDa±3 kDa and 75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P04004

• Expression Region:

  Asp 20 - Leu 478

• Host:

  HEK293

• Target:

  Vitronectin

• Conjugation:

  Unconjugated

• Tag:

  Native

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  0.01 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human Vitronectin Protein, Tag Free (Cat. No. VIN-H5214) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82W3) with a linear range of 0.002-0.156 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  52.3 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Human