Dog CTXII (Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit

CAT:
855-ELK10021-02
Size:
96 Tests
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Dog CTXII (Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit - image 1

Dog CTXII (Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit

  • Description:

    This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog CTXII protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CTXII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CTXII in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Product Name Alternative:

    CTX-II; CTX2
  • Gene Aliases:

    CTX-II; CTX2
  • Reactivity:

    Dog
  • Field of Research:

    Metabolic pathway, Bone metabolism, Rheumatology
  • Assay Type:

    Competitive Inhibition
  • Assay Principle:

    This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog CTXII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CTXII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CTXII in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Assay Performance Time:

    2 H
  • Standard:

    10000 pg/mL
  • Sample Type:

    Serum, plasma, cell lysates, cell culture supernates and other biological fluids
  • Detection Range:

    156.25 - 10000 pg/mL
  • Sensitivity:

    53.5 pg/mL
  • Species:

    Dog
  • CAS Number:

    7732-18-5