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Recombinant Human Biotinidase (BTD), partial

CAT: 0399-CSB-EP002854HU1-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP002854HU1-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
(Biotinase)
Abbreviation
Recombinant Human BTD protein, partial
Gene Name
BTD
UniProt
P43251
Expression Region
322-397aa
Organism
Homo sapiens (Human)
Target Sequence
YHDMENPKSHLIIAQVAKNPVGLIGAENATGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNAPPTFHSE
Tag
N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Metabolism
Relevance
Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
41.3 kDa
References & Citations
"Partial biotinidase deficiency is usually due to the D444H mutation in the biotinidase gene." Swango K.L., Demirkol M., Huener G., Pronicka E., Sykut-Cegielska J., Schulze A., Mayatepek E., Wolf B. Hum. Genet. 102:571-575 (1998)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial

UniProtKB · P43251

Biotinidase

BTD_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43251
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BTD
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Biotinase
EC number
3.5.1.12
Processing
Precursor
Secondary accessions
A6NHF2, B2R865, B4DFX1, B4DLJ9, B7Z7C9, F8W1Q3, Q96EM9
Protein keywords

Coding sequence diversity

Alternative splicing

Technical term

Direct protein sequencingProteomics identificationReference proteome

Disease

Disease variant

PTM

Glycoprotein

Molecular function

Hydrolase

Cellular component

Secreted

Domain

Signal

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