Recombinant Plasmodium knowlesi Circumsporozoite protein

Recombinant Plasmodium knowlesi Circumsporozoite protein

• CAS Number: 9000-83-3
• UniProt: P02894
• Expression Region: 20-363aa
• Organism: Plasmodium knowlesi (strain H)
• Target Sequence: THFEHNVDLSRAINVNGVSFNNVDTSSLGAQQVRQSASRGRGLGEKPKEGADKEKKKEKGKEKEEEPKKPNENKLKQPNEGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANAGQPQAQGDGANVPRQGRNGGGAPAGGNEGNKQAGKGQGQNNQGANAPNEKVVNDYLHKIRSSVTTEWTPCSVTCGNGVRIRRKAHAGNKKAEDLTMDDLEVEACVMDKCAGIFNVVSNSLGLVILLVLALFN
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: Developed Protein
• Relevance: Essential sporozoite protein. In the mosquito vector, required for sporozoite development in the oocyst, migration through the vector hemolymph and entry into the vector salivary glands. In the vertebrate host, required for sporozoite migration through the host dermis and infection of host hepatocytes. Binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes.; [Circumsporozoite protein C-terminus]: In the vertebrate host, binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes and is required for sporozoite invasion of the host hepatocytes.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length of Mature Protein
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 42.2 kDa
• References & Citations: "Structure of the plasmodium knowlesi gene coding for the circumsporozoite protein." Ozaki L.S., Svec P., Nussenzweig R.S., Nussenzweig V., Godson G.N. Cell 34:815-822 (1983)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.