PE Conjugated Mouse CD16/32 Recombinant Rabbit Monoclonal Antibody [PSH06-60]

PE Conjugated Mouse CD16/32 Recombinant Rabbit Monoclonal Antibody [PSH06-60]

• Description: All of the Fcγ receptors (FcγR) belong to the immunoglobulin superfamily and are the most important Fc receptors for inducing phagocytosis of opsonized (marked) microbes. This family includes several members, FcγRI (CD64), FcγRIIA (CD32), FcγRIIB (CD32), FcγRIIIA (CD16a), FcγRIIIB (CD16b), which differ in their antibody affinities due to their different molecular structure. For instance, FcγRI binds to IgG more strongly than FcγRII or FcγRIII does. FcγRI also has an extracellular portion composed of three immunoglobulin (Ig)-like domains, one more domain than FcγRII or FcγRIII has. This property allows FcγRI to bind a sole IgG molecule (or monomer), but all Fcγ receptors must bind multiple IgG molecules within an immune complex to be activated. The Fc-gamma receptors differ in their affinity for IgG and likewise the different IgG subclasses have unique affinities for each of the Fc gamma receptors. These interactions are further tuned by the glycan (oligosaccharide) at position CH2-84.4 of IgG. For example, by creating steric hindrance, fucose containing CH2-84.4 glycans reduce IgG affinity for FcγRIIIA. In contrast, G0 glycans, which lack galactose and terminate instead with GlcNAc moieties, have increased affinity for FcγRIIIA.
• Product Name Alternative: CD16a antibody CD16b antibody CD32 antibody CD32A antibody CD32B antibody CDw32 antibody Fc fragment of IgG low affinity IIa receptor antibody Fc fragment of IgG low affinity IIb receptor antibody Fc fragment of IgG low affinity IIIa receptor antibody Fc fragment of IgG low affinity IIIb receptor antibody Fc gamma receptor III A antibody Fc gamma receptor IIIb antibody Fc gamma RIIa antibody Fc gamma RIIb antibody Fc gamma RIII beta antibody Fc-gamma RII-a antibody Fc-gamma-RIIa antibody FCG2A_HUMAN antibody FcGR antibody FCGR2A antibody FCGR2B antibody FCGR3 antibody FCGR3A antibody FCGR3B antibody FcRII-a antibody FcRIII antibody IgG Fc receptor II-a antibody Low affinity immunoglobulin gamma Fc region receptor II-a antibody
• Abbreviation: Fcgr3, CD16, Fcgr2, Fcgr2b, Ly-17, CD32
• CAS Number: 9000-83-3
• Swiss Prot: P08101 Mouse, P08508 Mouse, Q5D5I8 Mouse, Q5D5J5 Mouse, Q7TMW9 Mouse, Q9ES92 Mouse
• Cellular Locus: Cell membrane.
• Host: Rabbit
• Species Reactivity: Mouse
• Immunogen: Recombinant protein within
• Isotype: IgG
• Clone: PSH06-60
• Conjugation: PE
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: FC
• Positive Control: Mouse spleen cells.
• Dilution: FC: 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
• Molecular Weight: Predicted band size: 30/37 kDa
• Storage Conditions: Store at 2°C to 8°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes