Nicotinamide N-methyltransferase Inhibitor Screening Kit II (F)

• Description:

  Nicotinamide N-methyltransferase (NNMT; EC 2.1.1.1) is an important cytosolic enzyme. It catalyzes the N-methylation of nicotinamide by transferring a methyl group from S-adenosyl-L-methionine (SAM) to nicotinamide resulting in the formation of 1-methyl-nicotinamide (MNA) and S-adenosyl-L-homocysteine. NNMT plays a significant role in the regulation of metabolic pathways in tissues such as adipose and liver as well as in cancer cells. Abnormal NNMT expression and activity has been implicated in a number of chronic diseases including cardiovascular diseases, cancer, obestity, diabetes, osteoarthritis, Parkinson’s disease etc. Therefore, small molecule inhibitors of NNMT could be beneficial in developing therapeutics for diseases characterized by abnormal NNMT activity. BioVision’s Nicotinamide N-methyltransferase inhibitor screening Kit II is a non-coupled, plate-based fluorometric assay. The assay utilizes a selective NNMT substrate that is methylated by SAM to generate a fluorescent product, measured at Ex/Em = 320/420 nm. In the presence of 1-Methylnicotinamide, a NNMT inhibitor, the enzymatic activity is inhibited thereby resulting in a reduced fluorescent signal. The kit provids a one-step, rapid, reliable test for high-throughput screening of NNMT inhibitors.

• Reactivity:

  Mammalian

• Applications:

  For screening, studying and characterizing potential inhibitors of NNMT

• Detection Method:

  Fluorescence (Ex/Em = 320/420 nm)

• Features & Benefits:

  A non-coupled, plate-based fluorometric assay. A one-step, rapid, reliable test for high-throughput screening of NNMT inhibitors.

• Shipping Conditions:

  Gel Pack

• Storage Conditions:

  -20°C

• Shelf Life:

  12 months