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3C Protease

CAT: 1220-YVV07701Size: 5000 UDry Ice: NoHazardous: No
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CAT#:1220-YVV07701Size:5000 U
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Expression System
E.coli
Nature
Recombinant
Applications
Remove fusion tags from proteins with the HRV 3C cleavage sequence (LEVLFQ↓GP) .
Stability
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Endotoxin
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Bioactivity
One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.
Form
Liquid
Buffer
50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Notes
For research use only.
Species
HRV-14

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