3C Protease

• Description :

  3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.

• Expression System :

  E.coli

• Nature :

  Recombinant

• Applications :

  Remove fusion tags from proteins with the HRV 3C cleavage sequence (LEVLFQ↓GP) .

• Stability :

  Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

• Endotoxin :

  Please contact with the lab for this information.

• Purity :

  >95% as determined by SDS-PAGE.

• Bioactivity :

  One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.

• Form :

  Liquid

• Buffer :

  50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol

• Shipping Conditions :

  In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

• Notes :

  For research use only.

• Species :

  HRV-14