Anti-Intercalated DNA Mouse Monoclonal Antibody - Library Pack

• Description:

  Selection of Mouse Monoclonal antibodies specific to Intercalated DNA

• Product Name Alternative:

  Anti-Intercalated DNA Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:
  
  12401 - Anti-Intercalated DNA Mouse Monoclonal Antibody - Clone ET602.2 - Isotype IgG2b
  12402 - Anti-Intercalated DNA Mouse Monoclonal Antibody - Clone ET749.4 - Isotype IgG1
  

• Host:

  Mouse

• Reactivity:

  Eukaryotic

• Immunogen:

  Ethidium bromide intercalated calf thymus DNA.

• Target:

  Intercalated DNA

• Clonality:

  Monoclonal

• Type:

  Antibody Library Pack

• Applications:

  ELISA

• Field of Research:

  DNA

• Purification Method:

  Purified by Protein G affinity chromatography

• Concentration:

  Lot Specific

• Dilution:

  Dilute in PBS or medium that is identical to that used in the assay system.

• Format:

  Purified

• Form:

  Liquid

• Buffer:

  Phosphate Buffered Saline

• Additionnal Information:

  These antibodies may be used in ELISA to detect and quantitate intercalated DNA.

• Storage Conditions:

  These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

• Specificity:

  These antibodies specifically recognize intercalated eukaryotic DNA and do not cross-react with single or double-stranded non-intercalated DNA or with ethidium bromide.

• Formulation:

  PBS, pH 7.4.

• Buffer pH:

  pH 7.4

• Target Background:

  Ethidium bromide (EB) found fame in the late 1940s as an antitrypanosomal, antimicrobial, antibacterial, and antiviral agent. Its biological effects are a direct consequence of the inhibition of nucleic acid synthesis, which in turn is related to the specific binding of the drug to DNA. EB inhibits DNA polymerase and binds in vitro to both RNA and DNA. Investigation into the precise nature of the DNA-EB binding mechanism led to the discovery that EB binds by a mechanism termed intercalation. This process has been studied extensively during the past three decades, and the photophysical changes that accompany intercalation have been successfully applied to quantitate and structurally elucidate DNA. More relevant to the use of EB in molecular biology are the observed variations in its fluorescent characteristics on binding to polynucleotides.

• CAS Number:

  9007-83-4