Anti-PMS2 Antibody

Anti-PMS2 Antibody

• Description: Mouse Monoclonal Antibody specific to PMS2
• Product Name Alternative: DNA mismatch repair protein PMS2, PMS1 protein homolog 2
• CAS Number: 9007-83-4
• Gene Name: PMS2
• NCBI Gene ID: PMS2
• UniProt: P54278
• Cellular Locus: Nucleus
• Host: Mouse
• Reactivity: Human
• Immunogen: Recombinant human PMS2
• Target Antigen: Mismatch repair endonuclease PMS2
• Target: PMS2
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: IHC
• Field of Research: Cancer research
• Purification Method: Purified by immunoaffinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Tris
• Function: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. {PubMed:16873062, PubMed:18206974, PubMed:23709753}.
• Additionnal Information:
  
  Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
• Storage Conditions: Store at 2-8°C. Do not freeze.
• Specificity: Human PMS2. Reactivity with other species has not been investigated.
• Formulation: Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
• Buffer pH: pH 7.3-7.7