Mouse anti-Histone H3 (TriMethyl K9) Antibody

• Background:

  Mouse monoclonal antibody to Histone H3 (TriMethyl K9)

• Synonyms:

  HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

• Host:

  Mouse

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence of human Histone H3. The exact sequence is proprietary.

• Clonality:

  Monoclonal

• Applications:

  WB, IHC, IP

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/1000 - 1/3000), IH (1/200 - 1/500), IP (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Histone H3 (TriMethyl K9) expression in Hela (A) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Histone H3 (TriMethyl K9) staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IP description:

  Immunoprecipitation of Histone H3 (TriMethyl K9) from 0.5mg Hela whole cell extract lysate, using anti-Histone H3 (TriMethyl K9) Antibody.

• Gene ID (Human):

  8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968

• Gene ID (Mouse):

  319152; 15077; 15078

• Gene ID (Rat):

  291159; 100361558

• SwissProt (Human):

  P68431; Q71DI3; P84243

• SwissProt (Mouse):

  P68433; P84228; P84244

• SwissProt (Rat):

  Q6LED0; P84245

• CAS Number:

  9007-83-4