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Mouse anti-eIF4AI Antibody

CAT: 0946-YLD8437-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0946-YLD8437-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mouse monoclonal antibody to eIF4AI
CAS Number
9007-83-4
Synonyms
DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human eIF4AI. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC, IF/IC
Concentration
1mg/mL
Dilution
WB (1/1000 - 1/3000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Mouse
WB Description
Western blot analysis of eIF4AI expression in 293T (A), Hela (B), HepG2 (C), mouse brain (D) whole cell lysates.
IHC description
Immunohistochemical analysis of eIF4AI staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of eIF4AI staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
1973
Gene ID (Mouse)
13681
SwissProt (Human)
P60842
SwissProt (Mouse)
P60843

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