Rabbit anti-Tryptase alpha/beta Antibody

• Background:

  Rabbit polyclonal antibody to Tryptase alpha/beta

• Synonyms:

  TPS1; TPS2; TPSB1; Tryptase alpha/beta-1; Tryptase-1; Tryptase I; Tryptase alpha-1

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human Tryptase alpha/beta

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Tryptase alpha/beta expression in HepG2 (A), mouse brain (B), mouse liver (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of Tryptase alpha/beta staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  7177

• Gene ID (Mouse):

  100503895

• Gene ID (Rat):

  54271

• SwissProt (Human):

  Q15661

• SwissProt (Mouse):

  Q02844

• SwissProt (Rat):

  P27435

• CAS Number:

  9007-83-4